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ROS detection, Intracellular ,ROS, generation was assessed using an ,ROS, assay ,kit, according to the manufacturer’s instructions. In vivo tumor xenograft study Three to four week old BALB/c homozygous (nu/nu) nude mice with 18-20 gbody weight were purchased from ,Shanghai, SLAC Laboratory Animal Co Ltd. (,Shanghai,, ,Shanghai,, China). The mice were
potential ,detection kit, (Beyotime, ,Shanghai,, China) according to the manufacturer’s protocols and the methods described previously . 2.6. Determination of the Intracellular ,ROS, The intracellular ,ROS, levels were determined by using a ,ROS, Assay ,Kit, (Beyotime, ,Shanghai,, China) following the protocols and the methods described previously .
werefromCorning,,Shanghai,,China.6-carboxy-2′,7′ dichloro-dihydro-ﬂuorescein diacetate (DCFH-DA) ,ROS detection kit, was purchased from Beyotime Institute of Biotechnology, Haimen, Jiangsu, China. MDA ,detection kit, was obtained from Nanjing Jiancheng bioengineering institute, Nanjing, China. The standard reagents with chromatographic purity
different post-infection time points by using GSH and GSSH assay ,kits, (Beyotime, ,Shanghai,, China) in accordance with the manufacturer’s instructions to determine the cellular antioxidant activity. 2.6. ,ROS Detection, The control cells and the infected A549 cells were washed twice with pre-warmed phosphate-buffered saline (PBS).
The mitochondrial protein mitoNEET is a type of iron-sulfur protein localized to the outer membrane of mitochondria and is involved in a variety of human pathologies including cystic fibrosis, diabetes, muscle atrophy, and neurodegeneration. In the current study, we found that isoliquiritigenin (ISL), one of the components of the root of >Glycyrrhiza glabra L., could decrease the ...
detection kit, (Nanjing Jiancheng Biomedical Engineering Co. Ltd., Nanjing, China). The ,ROS, levels in rat serum and kidney tissues were detected using the ,ROS detection kit, (,Shanghai, Yueyan Biological Technology Co., Ltd., ,Shanghai,, China). All procedures were per-formed in strict accordance with the ,kit, …
Background: Graphene and its derivative graphene oxide (GO) have been implicated in a wide range of anticancer effects. Purpose: The objective of this study was to systematically evaluate the toxicity and underlying mechanisms of GO on two osteosarcoma (OSA) cancer cell lines, MG-63 and K 7 M 2 cells. Methods: MG-63 and K 7 M 2 cells were treated by GO (0-50 µg/mL) for various time periods.